Архив 15 Январь 2010

Colombo, R.; F. D’Angelo; A. Arzini; F. Abbritti & L. Boccalon
Via Alberto da Giussano, 8, 20025, Legnano (MI), Italy
Gazzetta Medica Italiana Archivio per le Scienze Mediche 159(2): 53-57, 2000.
Article written in Italian
ISSN: 0393-3660
CAS REGISTRY NUMBER: 7782-44-7
Abstract
Background
The therapeutic effects of intravenous ozone in the treatment of arterial occlusive disease have been known for some time. Various authors have shown how this treatment improves the transport and release of oxygen to tissues.
Methods: Among the main mechanisms underlying this result, the most important is increased 2,3 diphosphoglycerate (2,3 DPG). This enzyme results in a diminished affinity of the hemoglobin for oxygen, thereby enabling the latter’s increased release to the tissues. Results: We compared the results in a sample of patients receiving conventional pharmacological therapy and a group of patients receiving ozone therapy according to our usual protocols. All the patients were treated using a major own-blood transfusion. The dose used for every transfusion was 5000 ng ozone added to 200 cc uncoagulated blood with citrate. Applications were continued twice weekly for a total of 5 sessions. A total of 50 pharmacologically-treated patients were included at Fontaine’s second stage. Another group of 50 patients commenced ozone treatment at the same time. Patients receiving pharmacological treatment presented improved symptoms in 50% of cases. Those who were treated with ozone treatment showed improved symptoms in 86% of cases. Changes in 2,3 DPG evaluated before and after treatment were correlated with the improved symptoms achieved. In general low indices of 2,3 DPG corresponded to enhanced levels after treatment and improved symptoms. However, significant improvements were noted even in the event of high enzyme levels before treatment.
Conclusions:
The predictive criterion for cases with low indices of 2,3 DPG at the start of treatment is indicative and not absolute. Ozone activity induces improvements that exceed all expectations when the patients to be treated present a metabolic reserve requiring stimulation and strengthening.

Chang JD, Lu HS, Chang YF, Wang D.

Department of Orthopaedics, Johnson General Hospital, 425 Chang Tsau Road, Changhua, Taiwan ROC. jonhsonchangmd@yahoo.com.tw

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by invasion of hyperplastic synovial cells and progressive joint destruction. Ozone therapy has been proposed as an immunomodulator and cellular metabolic activator which shows long-term anti-inflammatory effects and serves to reduce further the proinflammatory factors. We purified RA synovial fibroblast cells (RA-SFc) from patients and avoided contaminating macrophages by flow cytometry, then treated them with ozone. Following the observable decreased production of proinflammatory factors TNF-alpha, IL-1beta, and IL-6 from RA-SFc, we infused the cultured RA-SFc into joints of severe combined immunodeficiency mice. The mRNA and protein levels of the RA-SFc exposed to 3% and 5% ozone were the same. As a result, 3% and 5% ozone applied externally ameliorated the inflammatory reaction of RA without toxicity or serious side effects. Therefore, ozone injected into the knees of RA patients could become a valuable treatment, and we confirm the interactive mechanism between ozone and RA-SFc.

Bhalla DK, Reinhart PG, Bai C, Gupta SK

Department of Occupational and Environmental Health Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA. ad6268@wayne.eduOzone

Ozone(O(3)) is a significant component of atmospheric air pollution and produces detrimental effects in the lung. Although the mechanism of O(3)-induced lung inflammation and injury is unclear, the increased release of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) by lung cells following O(3) exposure may shed some light on this subject. To investigate the role of TNF-alpha in the O(3)-induced pulmonary insult, we intraperitoneally injected rats with either rabbit preimmune serum or rabbit antirat TNF-alpha 1 h prior to O(3) exposure. Approximately 12 h after the end of O(3) exposure the animals were sacrificed, the lungs lavaged, and tissue samples collected for expression of cytokine genes relevant to inflammation. The bronchoalveolar lavage fluid (BALF) was analyzed for albumin as a marker of pulmonary epithelial permeability changes and for fibronectin for its role in lung injury and repair. The lavage cells were collected, counted, and identified to quantitate the inflammatory response. Ozone exposure resulted in a significant increase in BALF albumin and fibronectin as compared to air-exposed controls and a significant increase in BALF polymorphonuclear leukocytes (PMNs). Antibody treatment produced a significant decrease in BALF albumin and PMNs as compared to O(3)-exposed rats given preimmune serum. Antibody treatment did not affect the BALF fibronectin concentration or the total cell count in the BAL. Tissue analysis for gene arrays revealed an activation of IL-1alpha, IL-6, and IL-10 in animals exposed to O(3). The gene expression was downregulated in animals treated with anti-TNF-alpha antibody prior to O(3) exposure. The results suggest a central role for TNF-alpha in the mechanistic pathways critical to lung inflammation. The significance of TNF-alpha in the inflammation and epithelial injury produced by ozone exposure reflects its overall contribution through modulation of other cytokines.

Ernst E.

School of Postgraduate Medicine and Health Sciences, University of Exeter, UK. E.Ernst@exeter.ac.ukComplementary and alternative medicine (CAM) is frequently used by cancer patients, and many oncologists have limited knowledge of CAM. This article provides a brief, evidence-based introduction to several CAM treatments relevant in the context of cancer. “Alternative” diets, chiropractic, coffee enemas, ozone therapy, and shark cartilage seem to have little to offer cancer patients. The evidence for or against homoeopathy and spiritual healing is at present inconclusive. Acupuncture, aromatherapy, and meditation may be useful for nausea/vomiting, for mild relaxation, and for pain/anxiety, respectively. Herbal treatments offer no reasonable prospect of a cure (mistletoe), but could be useful as palliative treatments (eg, for depression [St John's wort] or anxiety [kava]). Our knowledge regarding the potential benefit and harm of CAM is insufficient

Ros J.M., Grangeat A., Prez A. and Croce E.
(Argentina)
The treatment of the acute herniation disk can be approached through various methods, some of them being non-surgical like discolisis with oxygen-ozone therapy (O2-O3). The aim of this paper is to analyse the progressive changes occurring in the RMI and the absence of correlation between the former and the clinical improvement of the symptoms in patients undergoing ozone therapy due to acute disk herniation with root compression. Between April 2002 and December 2003, 122 patients with herniation disc pathology underwent oxygen-ozone therapy discolysis. Fourty of them with acute herniation lumbar disk, of whom 28 (70 %) were male and 12 female (30 %). Mean age: 36.89. A hundred percent complained about pain which resulted in: social behaviour changes 30 (75 %), wakefulness 28 (70 %), amusement disturbances 20 (50 %), sexual disturbances 16 (40 %). Other minor disorders were sensory changes like disesthesia, paresthesia, or anaesthesia). Radiology diagnosis was utilised in all cases by means of RMI. Twenty two out of 40 (55 %) had involved L5-S1 space. Heigth (20 %) L4-L5, 6 (15 %) L3-L4, and 4 (10%) had more than one level involved. Test on pain and influence on various aspects were run, which resulted in a pain index inability (IDD). Both RMI and IDD results were later compared. These patients underwent 10 sessions of O2-O3, two of which consisted of 6 mg/dose and 8 of 30 mg/dose. After the treatment, further tests were performed and new IDD results were obtained. These results were later classified as follows: Excellent (35 %); Very Good (50 %); Good (10 %), fairly good (5%) and failure (0%). In fact, ozone-therapy results in an improvement in the disc-nerve root conflict and in the overall clinical picture. Such achievement is independent of the mechanical factor changes, which will take place only later in time (6 – 12 months). It can be clearly observed that patients have improved their symptoms independently of the RMI changes, which occur only six month after ending the treatment. Pain resolution and its concomitant disabilities is the aim of the treatment in patients with acute disc herniation.

S. Sorokina, M. Zaslavskaja.The Medical Academy of Nizhni Novgorod, RussiaThe problem of paradontium inflammatory diseases treatment is one of the most urgent problems in stomatology. In spite of an abundance of existing remedies and methods of treatment this problem is far from solution. It was noted periodontal pocket microflora accustomization to antibacterial preparations and allergic reactions and side effects to them. The importance of searching new remedies becomes evident. The use of ozonized solutions for complex treatment of inflammatory diseases of paradontium is of great interest. For microbiological experiment a single irrigation of pathological periodontal pockets with ozonized solutions (water, olive oil) was performed. The pocket contents before and after irrigation were inoculated on a blood agar. Microscopic specimens were prepared simultaneously. In 18-20 hours the grown colonies were counted. After comparison of the obtained results a substantial antibacterial activity of ozonized solutions was revealed. Number of colonies before irrigation was 48.65.1 and after irrigation it was 6.2 0.3. the specimens show a significant decrease and qualitative changes of pathological pockets contents.??

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