Ozone therapy effects in the oxidative stress associated to diabetes mellitus

Al-Dalien S.M.1, MenГ©ndez S.2, MartГ­nez G.1, FernГЎndez-MontequГ­n J.I.3, Candelario E.J.1 and LeГіn O.S.1.
1Center for Research and Biological Evaluation, University of Havana, Institute of Pharmacy and Food Sciences, Cuba.
2Ozone Research Center, Cuba.
3Institute of Angiology and Vascular Surgery, Cuba.
ABSTRACT
It is well recognized the presence of oxidative stress in diabetes mellitus. Ozone can exert its protective effects by means of an oxidative preconditioning, stimulating and/or preserving the endogenous antioxidant systems. The aim of this paper is to evaluate the ozone effects, in a preclinical and preliminary clinical studies, in the oxidative stress associated to diabetes. Rats were divided in: 1- negative control group; 2- positive, using streptozotocin (STZ) as a diabetes inductor; 3- ozone, 10 treatments (1 mg kg-1), after STZ-induced diabetes and 4-oxygen (26 mg kg-1), as group 3 but using oxygen. Patients with diabetic foot were divided in 2 groups: ozone (using rectal and local ozone) and antibiotic (systemic and locally). Ozone treatment improved glycemic control and prevented oxidative stress associated to diabetes mellitus and its complications, in both studies, in agreement with the excellent results obtained clinically.