Asiatic-European association of ozone therapists

Bhalla DK, Reinhart PG, Bai C, Gupta SK

Department of Occupational and Environmental Health Sciences, College of Pharmacy and Health Sciences, Wayne State University, Detroit, MI 48202, USA. ad6268@wayne.eduOzone

Ozone(O(3)) is a significant component of atmospheric air pollution and produces detrimental effects in the lung. Although the mechanism of O(3)-induced lung inflammation and injury is unclear, the increased release of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) by lung cells following O(3) exposure may shed some light on this subject. To investigate the role of TNF-alpha in the O(3)-induced pulmonary insult, we intraperitoneally injected rats with either rabbit preimmune serum or rabbit antirat TNF-alpha 1 h prior to O(3) exposure. Approximately 12 h after the end of O(3) exposure the animals were sacrificed, the lungs lavaged, and tissue samples collected for expression of cytokine genes relevant to inflammation. The bronchoalveolar lavage fluid (BALF) was analyzed for albumin as a marker of pulmonary epithelial permeability changes and for fibronectin for its role in lung injury and repair. The lavage cells were collected, counted, and identified to quantitate the inflammatory response. Ozone exposure resulted in a significant increase in BALF albumin and fibronectin as compared to air-exposed controls and a significant increase in BALF polymorphonuclear leukocytes (PMNs). Antibody treatment produced a significant decrease in BALF albumin and PMNs as compared to O(3)-exposed rats given preimmune serum. Antibody treatment did not affect the BALF fibronectin concentration or the total cell count in the BAL. Tissue analysis for gene arrays revealed an activation of IL-1alpha, IL-6, and IL-10 in animals exposed to O(3). The gene expression was downregulated in animals treated with anti-TNF-alpha antibody prior to O(3) exposure. The results suggest a central role for TNF-alpha in the mechanistic pathways critical to lung inflammation. The significance of TNF-alpha in the inflammation and epithelial injury produced by ozone exposure reflects its overall contribution through modulation of other cytokines.

Ernst E.

School of Postgraduate Medicine and Health Sciences, University of Exeter, UK. E.Ernst@exeter.ac.ukComplementary and alternative medicine (CAM) is frequently used by cancer patients, and many oncologists have limited knowledge of CAM. This article provides a brief, evidence-based introduction to several CAM treatments relevant in the context of cancer. “Alternative” diets, chiropractic, coffee enemas, ozone therapy, and shark cartilage seem to have little to offer cancer patients. The evidence for or against homoeopathy and spiritual healing is at present inconclusive. Acupuncture, aromatherapy, and meditation may be useful for nausea/vomiting, for mild relaxation, and for pain/anxiety, respectively. Herbal treatments offer no reasonable prospect of a cure (mistletoe), but could be useful as palliative treatments (eg, for depression [St John’s wort] or anxiety [kava]). Our knowledge regarding the potential benefit and harm of CAM is insufficient

Ros J.M., Grangeat A., Prez A. and Croce E.
(Argentina)
The treatment of the acute herniation disk can be approached through various methods, some of them being non-surgical like discolisis with oxygen-ozone therapy (O2-O3). The aim of this paper is to analyse the progressive changes occurring in the RMI and the absence of correlation between the former and the clinical improvement of the symptoms in patients undergoing ozone therapy due to acute disk herniation with root compression. Between April 2002 and December 2003, 122 patients with herniation disc pathology underwent oxygen-ozone therapy discolysis. Fourty of them with acute herniation lumbar disk, of whom 28 (70 %) were male and 12 female (30 %). Mean age: 36.89. A hundred percent complained about pain which resulted in: social behaviour changes 30 (75 %), wakefulness 28 (70 %), amusement disturbances 20 (50 %), sexual disturbances 16 (40 %). Other minor disorders were sensory changes like disesthesia, paresthesia, or anaesthesia). Radiology diagnosis was utilised in all cases by means of RMI. Twenty two out of 40 (55 %) had involved L5-S1 space. Heigth (20 %) L4-L5, 6 (15 %) L3-L4, and 4 (10%) had more than one level involved. Test on pain and influence on various aspects were run, which resulted in a pain index inability (IDD). Both RMI and IDD results were later compared. These patients underwent 10 sessions of O2-O3, two of which consisted of 6 mg/dose and 8 of 30 mg/dose. After the treatment, further tests were performed and new IDD results were obtained. These results were later classified as follows: Excellent (35 %); Very Good (50 %); Good (10 %), fairly good (5%) and failure (0%). In fact, ozone-therapy results in an improvement in the disc-nerve root conflict and in the overall clinical picture. Such achievement is independent of the mechanical factor changes, which will take place only later in time (6 - 12 months). It can be clearly observed that patients have improved their symptoms independently of the RMI changes, which occur only six month after ending the treatment. Pain resolution and its concomitant disabilities is the aim of the treatment in patients with acute disc herniation.

S. Sorokina, M. Zaslavskaja.The Medical Academy of Nizhni Novgorod, RussiaThe problem of paradontium inflammatory diseases treatment is one of the most urgent problems in stomatology. In spite of an abundance of existing remedies and methods of treatment this problem is far from solution. It was noted periodontal pocket microflora accustomization to antibacterial preparations and allergic reactions and side effects to them. The importance of searching new remedies becomes evident. The use of ozonized solutions for complex treatment of inflammatory diseases of paradontium is of great interest. For microbiological experiment a single irrigation of pathological periodontal pockets with ozonized solutions (water, olive oil) was performed. The pocket contents before and after irrigation were inoculated on a blood agar. Microscopic specimens were prepared simultaneously. In 18-20 hours the grown colonies were counted. After comparison of the obtained results a substantial antibacterial activity of ozonized solutions was revealed. Number of colonies before irrigation was 48.65.1 and after irrigation it was 6.2 0.3. the specimens show a significant decrease and qualitative changes of pathological pockets contents.??

V.V.Mekhedko, E.I.Nazarov
National Medical Academy of Postgraduate Education named after P.L.Shupyk,
All-Ukrainian association of ozone therapists and manufacturers of the equipment.
Research-and-production enterprise Econika, Ukraine, www.ozonetherapy.org
Prolapsus of internal genitals gives up to 15 % among indications to surgical treatment of women in gynecologic hospitals of Ukraine. The tactic peculiarity in management of patients in the postoperative period is prevention of inflammatory complications as the rate of such complications makes up to 25 %. Usually various antiseptics are applied for sanitation of vaginal postoperative wounds, but for today such preparations are not so effective, causing allergic reactions and irritations, and do not influence local immunity of a wound.
The aim of the work is to study the effect of the ozonized olive oil on local immunity of a vaginal postoperative wound in women in whom extirpation of the uterus through the vagina have been performed on the basis of laboratory investigations.
In order to study reparative processes in the vaginal postoperative wound and a condition of local immunity we took discharge from the vagina before surgery, then wound secretion was taken from the vagina on ??, , V, V day after surgery for determination the amount of secretory immunoglobulin A (SIgA), as well as washouts from the vagina before surgery and on the 7th day after surgery - for studying phagocytosis activity and a level of immunoglobulins A, M, G by a method of radial immunodiffusion by Manchini et.al. [1].
Depending on the method of treatment of the vaginal postoperative wound the patients have been divided into 2 groups. The first, basic group, consisted of 30 patients who were made applications of the ozonized olive oil to the vaginal wound prepared by barbotage of the olive oil with ozone-oxygen mixture with ozone concentration of 50 mg/l for 60 min. The second, control group, comprised 30 women who have been made sanitation of the vaginal postoperative wound by usual antiseptics.
While investigating a condition of vaginal local immunity of women before surgical intervention, we found out that the amount of immunoglobulins, a level of phagocytic number, phagocytic index, and values of Ig G, A, M did not differ in washouts from the vagina in both groups.
??In the basic group of women contents of Ig A became by 18 %, IgG?� by 9 % higher than in the control group on the 7th day after surgery with simultaneous reduction of IgM by 33 %. Phagocytic index of neutrophils in wound exudate was considerably higher in the basic group, against the background of application of the ozonized olive oil?�by 57 % in comparison with the control group, and phagocytic number was higher by 51 % accordingly Before surgical treatment the values of secretory immunoglobulin A did not differ essentially in both groups. Against the background of application of the ozonized olive oil to the vaginal wound the amount of SIgA after operation increased by 20 % in comparison with the control group on the 1st day, on the 3rd day - by13 %, on the 5th day - by22 % and on the 7th day - by 15 % ??onclusions.
?�1. We received confirmation of the positive effect of the ozonized olive oil on local immunity that had caused additional migration of immunocompetent cells in the postoperative wound.
2. Increased level of secretory immunoglobulin A in wound secretion which positively affected the course of the wound process confirms immunomodulating effect of the ozonized olive oil. Application of the given medical complex also had the expressed action on dynamics of phagocytic number and phagocytic index.
?�Thus, advantage of the method of treatment of vaginal postoperative wounds with the ozonized olive oil before treatment by traditional antiseptics consists in acceleration of reparative processes in the wound and immunostimulating effect. It allowed to recommend it for application in treatment of postoperative wounds in gynecologic departments.
The literature:
1. Mancini G., Carbonara A., Heremans I. Immunochemical quantitation of antigens by single radial immunodiffusion // Immunochemistry.?� 1965.?� V. 2. p. 235-254.