Asiatic-European association of ozone therapists

Turrent J.1 and Men?�ndez S.2.
1″Cira Garc?�a” Central Clinic, Cuba.
2Ozone Research Center, Cuba.
ABSTRACT
Ozone therapy is presented like an alternative therapy in critically ill patients. After ozone therapy, an improvement in the hemodynamic parameters with an increase in tissue oxygenation were demonstrated. No modifications in the cardiac output were reported. A modulating effect of ozone therapy in certain biochemical parameters was observed. Also, microbiologic cultures were negative and leukocyte figure became normal. An ozone therapy preventive effect in the appearance of generalized sepsis was observed. With all these results we conclude that ozone therapy can be considered as a prophylactic therapy in critically ill patients.

Zamora Z.B.1, Men?�ndez S.1, Bette M.2, Mutters R.3, Hoffmann S.4 and Schulz S.4.
1Ozone Research Center, Cuba.
2Institute of Anatomy Celular and Molecular Biology, University od Marburg, Germany.
3Intitute of Microbiology, University od Marburg, Germany.
4Institute of Animals Laboratory, University od Marburg, Germany.
192 Wistar male rats (180 - 200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model. The proinflammatory cytokines (IL-1b, TNFa, IL-6 and IL-2) expression were also determinated in different haemathopoyetic organs. The animals were taken at random and divided into 16 groups, 12 animals each: Group A, positive control (sepsis without treatment); groups B and C treated with ozone (200 mg / 250 g b. w) and Cefotaxim (25 mg/kg), (0h, +1h) and (+1 h) respectivitly; groups D and E, ozone and Cefodizim (25 mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9 %. The other groups (J-P) of animals were treated only with antibiotics.
The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1b mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism.

.Ajamieh H.H.1, Men????ndez S.2, Merino N.1, Sam S.1, Le??n O.S.1.
1Center of Research and Biological Evaluations. Food Pharmacy Institute. Havana University, Cuba.
2Ozone Research Center,Cuba????????????????????