Архив Июль 2007

GutiГ©rrez M.S., Lezcano I., Baluja Ch. and SГЎnchez E.
Ozone Research Center
, Cuba.
ABSTRACT
To determine the ozone inactivation kinetic of different Gram positive and Gram negative multiple antibiotics resistant bacterial strains from clinical isolation (CI), that resists too high concentration of chlorine, and compare them with reference strains was our objective. Microorganism’s concentration was from 105 to 106 ufc/mL, and chlorine and ozone concentrations were between 0.5 – 4 mg/L, and 0.36 – 2 mg/L respectively. Gram-positive multiple antibiotic resistant strains are the most resistant bacteria with chlorine. With ozone the multiple antibiotics resistant strains were more resistant that reference strains. There were not statistical differences on ozone resistant inside of strains of same species, neither between Gram

HernГЎndez F., Alvarez I., Corcho I. and GonzГЎlez M.
Ozone Research Center
, Cuba.
ABSTRACT
We have studied the behavior of the erythrocyte glutathione antioxidant pathway, the HLA-DR molecule expression and the serum IgE level in patients suffering reactive asthma, whose were treated with ozone major autohemotherapy or rectal insufflation. A subgroup of patients having high levels of serum IgE (> 250 IU/mL) presented high level of HLA-DR expression (> 35 %) and lower concentrations of erythrocyte GSH (< 2.00 Вµmol/g.Hb) and GPx (< 11.62 IU/g.Hb) as a basal state before the beginning of ozone therapy. GST and GR were in the normal range. After to complete 15 sessions of ozone autohemotherapy applications (dose of 8 mg each session), all parameters in the glutathione antioxidant pathway were highly elevated and, IgE and HLA-DR levels decreased markedly respect to the initial values. With ozone rectal insufflation (20 sessions at an ozone dose of 10 mg each one), similar results were achieved although the changes were not so significant as in authohemotherapy. When the analysis was done considering all patients (with or without initial high level of IgE) the changes in GSH, GPx and GST were sligthly higher after autohemotherapy without changes in GR and IgE, but HLA-DR expression significantly decreased. These findings support a role for IgE producing oxidative stress and inflammation in allergenic asthma. On the other hand, ozone therapy proved to be effective in this disease due, likely, to its regulation on glutathione antioxidant pathway which lead to a control in the oxidative stress present in the asthma patients and a change in the cytokine pattern.

Copello M.1, EguГ­a F.2, MenГ©ndez S.3 and MenГ©ndez N.1.
1Retinitis
Pigmentosa National Reference Center, “Dr. Salvador Allende” Hospital, Cuba.
2″RamГіn Pando Ferrer” Ophthalmologic Hospital,
Cuba.
3Ozone
Research Center, Cuba.
ABSTRACT

Retinitis pigmentosa (RP) is characterized by progressive night blindness. The aim of this study is to determine the efficacy of ozone therapy in patients with RP. A controlled, randomized, double blind clinical trial involving 68 patients was performed. Patients were divided into 2 groups: ozone, patients were treated with ozone by rectal administration (dose = 10 mg), during 15 sessions; control, as ozone group, but using oxygen. The main outcome variable was the visual field area (VFA). Results demonstrated a significant improvement (SI) in 88.2 % of patients treated with ozone in comparison with 23.5 % achieved in the control group. In the ozone group, VFA tend to stabilize beyond a mean time of 6.83 months with a loose in SI afterward. A temporal positive effect of ozone therapy, over the natural course of RP, was found. It could be useful to apply ozone therapy in the first stages of the disease and at six-month intervals in order to enhance visual capabilities in RP patients.

Al-Dalien S.M.1, MenГ©ndez S.2, MartГ­nez G.1, FernГЎndez-MontequГ­n J.I.3, Candelario E.J.1 and LeГіn O.S.1.
1Center for Research and Biological Evaluation, University of Havana, Institute of Pharmacy and Food Sciences, Cuba.
2Ozone Research Center, Cuba.
3Institute of Angiology and Vascular Surgery, Cuba.
ABSTRACT
It is well recognized the presence of oxidative stress in diabetes mellitus. Ozone can exert its protective effects by means of an oxidative preconditioning, stimulating and/or preserving the endogenous antioxidant systems. The aim of this paper is to evaluate the ozone effects, in a preclinical and preliminary clinical studies, in the oxidative stress associated to diabetes. Rats were divided in: 1- negative control group; 2- positive, using streptozotocin (STZ) as a diabetes inductor; 3- ozone, 10 treatments (1 mg kg-1), after STZ-induced diabetes and 4-oxygen (26 mg kg-1), as group 3 but using oxygen. Patients with diabetic foot were divided in 2 groups: ozone (using rectal and local ozone) and antibiotic (systemic and locally). Ozone treatment improved glycemic control and prevented oxidative stress associated to diabetes mellitus and its complications, in both studies, in agreement with the excellent results obtained clinically.

Turrent J.1 and MenГ©ndez S.2.
1″Cira GarcГ­a” Central Clinic,
Cuba
.
2Ozone
Research Center, Cuba.
ABSTRACT
Ozone therapy is presented like an alternative therapy in critically ill patients. After ozone therapy, an improvement in the hemodynamic parameters with an increase in tissue oxygenation were demonstrated. No modifications in the cardiac output were reported. A modulating effect of ozone therapy in certain biochemical parameters was observed. Also, microbiologic cultures were negative and leukocyte figure became normal. An ozone therapy preventive effect in the appearance of generalized sepsis was observed. With all these results we conclude that ozone therapy can be considered as a prophylactic therapy in critically ill patients.

Zamora Z.B.1, MenГ©ndez S.1, Bette M.2, Mutters R.3, Hoffmann S.4 and Schulz S.4.
1Ozone
Research Center, Cuba
.
2Institute of Anatomy Celular and Molecular Biology, University od
Marburg, Germany
.
3Intitute of Microbiology, University od
Marburg, Germany
.
4Institute of Animals Laboratory, University od
Marburg, Germany.
192 Wistar male rats (180 – 200 g) were used to evaluate the prophylactic effect of ozone combined with antibiotics in septic shock model. The proinflammatory cytokines (IL-1b, TNFa, IL-6 and IL-2) expression were also determinated in different haemathopoyetic organs. The animals were taken at random and divided into 16 groups, 12 animals each: Group A, positive control (sepsis without treatment); groups B and C treated with ozone (200 mg / 250 g b. w) and Cefotaxim (25 mg/kg), (0h, +1h) and (+1 h) respectivitly; groups D and E, ozone and Cefodizim (25 mg/kg (0h, +1h) and (+1 h) respectively; groups F and G ozone and levofloxacin (12.5 mg/kg), (0h, +1h) and (+1 h) respectively; groups H and I ozone and Piperacillin /Tazobactam (65mg/kg), (0h, +1h) and (+1 h) respectively. The septic shock was carried out by single intraperitoneal injection of fecal material (0.65 g/kg b/w) from a donor rat, diluted in saline solution 0.9 %. The other groups (J-P) of animals were treated only with antibiotics.
The group pretreated with ozone and antibiotics showed a significant increase in the survival rate and showed also a decrease of IL-1b mRNA expression in liver. Ozone pretreatment might prevent lethal peritonitis by controlling inflammatory process generated physiologically into the body against specific damage or specific organism
.

.Ajamieh H.H.1, Men????ndez S.2, Merino N.1, Sam S.1, Le??n O.S.1.
1Center of Research and Biological Evaluations. Food Pharmacy Institute.
Havana University, Cuba.
2Ozone
Research Center,Cuba
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It has been demonstrated that ozone, probably by means of an oxidative preconditioning (OP) mechanism, protected the liver against the damage mediated by reactive oxygen species. Taking into account that ischemic preconditioning (IschP) is also a protective mechanism, a comparative study between both preconditioning effects, with the aim to study the effectiveness of both protective procedures, was performed. Rats were divided at random in: 1- control, sham operated (anesthesia and laparotomy plus surgical manipulation); 2- I/R (ischemia for 90 min followed by 90 min reperfusion); 3- IschP + I/R, as group 2 but submitted to a previous ischemic preconditioning (ischemia 10 min and reperfusion 10 min); 4- OzoneOP + I/R, as group 2 but submitted to a previous oxidative preconditioning with 15 sessions, daily, of ozone by rectal administration (dose = 1 mg/kg). The comparison between both preconditionings showed no biochemical differences for the parameters evaluated. Nevertheless, the histological study demonstrated that the protective effect produced by the OzoneOP is superior to that achieved with the IschP.

Bataller M., Veliz E., Lodos D., Fernandez L.A. and Alvarez C.
Ozone Research Center,
Cuba.
The hydrodynamics study was conducted in a bubble column of a pilot plant. The proposed model considers the bubble column as the combination of a series of N equally sized CSTRs and a CSTR with a different sized respect to the series. The results indicated that the liquid flow rate influenced on the hydrodynamic behavior more than gas flow rate. Besides, the model was suitable to evaluate the hydrodynamic behavior of different columns in several ozonization plants in Cuba.

I. Corcho, F. HernГЎndez, N. Reyes, A. Carballo, T. Reyes1, L. Yanez1.
Ozone Research Center, Cuba.
1Betera Laboratory, Cuba.

The aim of this study was to asses the effect of ozone therapy on lymphocyte subpopulations CD3, CD4+, CD8+, leukocyte function CD45, HLA-DR molecule expression in the peripheral blood and the serum levels of IgE in patients with inflammatory diseases. Initial high values in the expression of HLA-DR molecule were significant decreased after endovenous ozone therapy sessions. A tendency to achieve normal values was observed in CD3, CD4+, CD8+ and CD45 parameters. These results suggest that ozone treatment can be a beneficial alternative therapy in inflammatory process.

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