Asiatic-European association of ozone therapists

Guti?�rrez M.S., Lezcano I., Baluja Ch. and S??nchez E.
Ozone Research Center, Cuba.
ABSTRACT
To determine the ozone inactivation kinetic of different Gram positive and Gram negative multiple antibiotics resistant bacterial strains from clinical isolation (CI), that resists too high concentration of chlorine, and compare them with reference strains was our objective. Microorganism’s concentration was from 105 to 106 ufc/mL, and chlorine and ozone concentrations were between 0.5 - 4 mg/L, and 0.36 - 2 mg/L respectively. Gram-positive multiple antibiotic resistant strains are the most resistant bacteria with chlorine. With ozone the multiple antibiotics resistant strains were more resistant that reference strains. There were not statistical differences on ozone resistant inside of strains of same species, neither between Gram

Hern??ndez F., Alvarez I., Corcho I. and Gonz??lez M.
Ozone Research Center, Cuba.
ABSTRACT
We have studied the behavior of the erythrocyte glutathione antioxidant pathway, the HLA-DR molecule expression and the serum IgE level in patients suffering reactive asthma, whose were treated with ozone major autohemotherapy or rectal insufflation. A subgroup of patients having high levels of serum IgE (> 250 IU/mL) presented high level of HLA-DR expression (> 35 %) and lower concentrations of erythrocyte GSH (< 2.00 ?�mol/g.Hb) and GPx (< 11.62 IU/g.Hb) as a basal state before the beginning of ozone therapy. GST and GR were in the normal range. After to complete 15 sessions of ozone autohemotherapy applications (dose of 8 mg each session), all parameters in the glutathione antioxidant pathway were highly elevated and, IgE and HLA-DR levels decreased markedly respect to the initial values. With ozone rectal insufflation (20 sessions at an ozone dose of 10 mg each one), similar results were achieved although the changes were not so significant as in authohemotherapy. When the analysis was done considering all patients (with or without initial high level of IgE) the changes in GSH, GPx and GST were sligthly higher after autohemotherapy without changes in GR and IgE, but HLA-DR expression significantly decreased. These findings support a role for IgE producing oxidative stress and inflammation in allergenic asthma. On the other hand, ozone therapy proved to be effective in this disease due, likely, to its regulation on glutathione antioxidant pathway which lead to a control in the oxidative stress present in the asthma patients and a change in the cytokine pattern.

Copello M.1, Egu?�a F.2, Men?�ndez S.3 and Men?�ndez N.1.
1Retinitis Pigmentosa National Reference Center, “Dr. Salvador Allende” Hospital, Cuba.
2″Ram??n Pando Ferrer” Ophthalmologic Hospital, Cuba.
3Ozone Research Center, Cuba.
ABSTRACT

Retinitis pigmentosa (RP) is characterized by progressive night blindness. The aim of this study is to determine the efficacy of ozone therapy in patients with RP. A controlled, randomized, double blind clinical trial involving 68 patients was performed. Patients were divided into 2 groups: ozone, patients were treated with ozone by rectal administration (dose = 10 mg), during 15 sessions; control, as ozone group, but using oxygen. The main outcome variable was the visual field area (VFA). Results demonstrated a significant improvement (SI) in 88.2 % of patients treated with ozone in comparison with 23.5 % achieved in the control group. In the ozone group, VFA tend to stabilize beyond a mean time of 6.83 months with a loose in SI afterward. A temporal positive effect of ozone therapy, over the natural course of RP, was found. It could be useful to apply ozone therapy in the first stages of the disease and at six-month intervals in order to enhance visual capabilities in RP patients.

Al-Dalien S.M.1, Men?�ndez S.2, Mart?�nez G.1, Fern??ndez-Montequ?�n J.I.3, Candelario E.J.1 and Le??n O.S.1.
1Center for Research and Biological Evaluation, University of Havana, Institute of Pharmacy and Food Sciences, Cuba.
2Ozone Research Center, Cuba.
3Institute of Angiology and Vascular Surgery, Cuba.
ABSTRACT
It is well recognized the presence of oxidative stress in diabetes mellitus. Ozone can exert its protective effects by means of an oxidative preconditioning, stimulating and/or preserving the endogenous antioxidant systems. The aim of this paper is to evaluate the ozone effects, in a preclinical and preliminary clinical studies, in the oxidative stress associated to diabetes. Rats were divided in: 1- negative control group; 2- positive, using streptozotocin (STZ) as a diabetes inductor; 3- ozone, 10 treatments (1 mg kg-1), after STZ-induced diabetes and 4-oxygen (26 mg kg-1), as group 3 but using oxygen. Patients with diabetic foot were divided in 2 groups: ozone (using rectal and local ozone) and antibiotic (systemic and locally). Ozone treatment improved glycemic control and prevented oxidative stress associated to diabetes mellitus and its complications, in both studies, in agreement with the excellent results obtained clinically.

Turrent J.1 and Men?�ndez S.2.
1″Cira Garc?�a” Central Clinic, Cuba.
2Ozone Research Center, Cuba.
ABSTRACT
Ozone therapy is presented like an alternative therapy in critically ill patients. After ozone therapy, an improvement in the hemodynamic parameters with an increase in tissue oxygenation were demonstrated. No modifications in the cardiac output were reported. A modulating effect of ozone therapy in certain biochemical parameters was observed. Also, microbiologic cultures were negative and leukocyte figure became normal. An ozone therapy preventive effect in the appearance of generalized sepsis was observed. With all these results we conclude that ozone therapy can be considered as a prophylactic therapy in critically ill patients.